RVT Test

RETROVIRAL SCREENIG TEST: Principle:-This test is used to screening persons who has come to know his / her status , this methodology is also used to test those products at high risk for the development of acquired immunodeficiency syndrome (AIDS) such as homosexual, intravenous drugs users, and other who have been exposed to unprotected sex, blood or blood product. Aim: to detect the presence of HIV/AIDS in blood sample. Materials Test Strip sample (serum / plasma/ whole blood) Buffer Gloves disposable pipette Procedure: The test strip was labeled with patient’s identification number. The protective foil cover of the strip was pulled off. About 50ul of sample was placed on the test strip using a Pasteur or precision pipette to the absorbent pad on the strip The result was observed after 15minutes.Results Interpretation:- Positive; two red/pink lines of any intensity appear in both the control and the patient areawhiletheNegative; one line appears in the control area and no line on the patient area Invalid; No line appears in the control area, if a line appears in the patient area or not. The test should be repeated with a new test device.